Coding

Part:BBa_K2332311:Design

Designed by: Camillo Moschner   Group: iGEM17_UCL   (2017-09-27)


PhoCl, a mammalian photocleavable protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 37
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 93
    Illegal XhoI site found at 102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 607


Design Notes

For easy expression we included the start codon inside the part. Furthermore, for easy extraction and analysis of the protein the part contains a His-tag followed by an enterokinase recognition and cleavage site.


Source

This part has been engineered by Zhang et al. 2017 (Robert E. Campbell lab). The Campbell lab has sent the original plasmid to the UCL iGEM 2017 as part of a collaboration between the lab and the iGEM team.

References